MLG have announced the groups for the last sixteen teams, that will face each other in a bid to secure one of the eight remaining spots at the upcoming MLG: Columbus 2016 Major tournament.
MLG have announced the groups for the final sixteen contenders that have passed through the minor qualification and last chance qualifier tournaments in the run-up to MLG: Columbus 2016. Upon conclusion of the qualifier, the top two teams from each group will move on to challenge the other eight teams that participated at the previous Major tournament. Said teams are below:
Team EnVyUs
Natus Vincere
FaZe Clan
Ninjas in Pyjamas
Fnatic
Virtus.Pro
Astralis
Luminosity Gaming
Following are the groups for the Main Qualifier:
| Group A | Group B | Group C | Group D |
| G2 Esports |  Mousesports |  Counter Logic Gaming | Cloud9 |
| Flipsid3 Tactics | Team Liquid | Vexed Gaming | Team Dignitas |
| Enemy | HellRaisers |  The Mongolz |  Gambit |
| Tempo Storm | Team YP | SK Gaming |  Renegades |
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